Getting Started in Cryo EM

90% Completion

DescriptionEnroll in Course

This Getting Started in Cryo EM course is designed to provide learners with a comprehensive introduction to the principles and techniques of cryo-electron microscopy (EM). Participants will gain a solid foundation in the theory and practical applications of cryo EM, enabling them to confidently pursue further studies or research in this field.

Syllabus

Lesson 1: Introduction to Cryo EM

• Understanding basics of Electron Microscopy
• Getting familiar with Cryo EM
• Significance of Cryo EM in scientific research
• Variants of Cryo EM: Single-particle analysis, Tomography, MicroED
• Understanding the role of Cryo EM in structural biology

Lesson 2: Equipment and Sample Preparation

• Key equipment used in Cryo EM
• Understanding Cryo EM Sample preparation
• Steps involved in Cryo EM sample preparation
• Common pitfalls in sample preparation and how to avoid them
• Maintaining Cryo conditions during EM sample preparation

Lesson 3: Image Acquisition and Analysis

• Understanding the architecture of a Cryo EM microscope
• Acquiring images with Cryo EM
• Analyzing Cryo EM images
• Software tools for Cryo EM image analysis
• Common challenges and troubleshooting tips in image acquisition and analysis

Lesson 4: Data Processing and Interpretation

• Introduction to Cryo EM data processing
• Understanding the process of model fitting and refinement
• Interpreting and validating Cryo EM data
• High resolution Cryo EM data
• Applying Cryo EM data in research

Lesson 5: Advances and Applications of Cryo EM

• Advances in Cryo EM technologies
• Applications of Cryo EM in structural biology
• Emerging trends in Cryo EM research
• Cryo EM in drug discovery and development
• The future of Cryo EM

Course Overview

The course will cover various topics related to cryo EM, including the fundamentals of electron microscopy, sample preparation techniques, image acquisition and processing, and structural analysis. Participants will also learn about the different types of cryo EM instruments and their functionalities. Through a combination of lectures, hands-on exercises, and case studies, learners will acquire essential knowledge and practical skills necessary to understand and utilize cryo EM in their own scientific endeavors.

Prerequisites

There are no specific prerequisites for this course. However, a basic understanding of biology and chemistry concepts would be beneficial for better comprehension of certain topics covered.

Skills Acquired

Upon completion of this course, learners will have acquired the following skills: 1. Knowledge of cryo EM principles and techniques. 2. Proficiency in sample preparation for cryo EM imaging. 3. Ability to analyze cryo EM images and process the data. 4. Understanding of different software tools utilized in cryo EM. 5. Familiarity with interpreting and presenting cryo EM data in a scientific context.

Resources

The course provides the following resources to facilitate learning: 1. Lecture slides and notes accessible through an online platform. 2. Reading materials, including scientific articles and publications related to cryo EM. 3. Hands-on exercises with cryo EM software and tools. 4. Case studies and real-world examples to reinforce concepts. 5. Discussion forums to interact with instructors and fellow learners. 6. Quizzes and assessments to evaluate progress and learning outcomes.